ARHGEF18 Knockout cell line (IPSC)

Loci	STR Info (Sample Cell) Sample Cell Line:IPSC-DYR0100			STR Info (Cell bank) Cell Line:ATCC- DYR0100 hiPSC
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	13		11	13
D7S820	9	10		9	10
D16S539	12	12		12	12
VWA	14	19		14	19
TH01	9.3	9.3		9.3	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	7	11		7	11
D12S391	18	19.3
FGA	21	22
D2S1338	17	19
D21S11	30	32.2
D18S51	10	13
D8S1179	14	15
D3S1358	15	17
D6S1043	12	13
PENTAE	7	7
D19S433	14	15
PENTAD	12	13
D1S1656	12	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARHGEF18 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC-DYR0100 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARHGEF18 gene knockout ARHGEF18 Knockout cell line（IPSC-DYR0100） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ARHGEF18 Knockout cell line (IPSC)

Catalog#

YKO-H985

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

ARHGEF18 Knockout cell line (Hep G2)
inquiry More details

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HT3981

YKO-HT3981(Hep G2)
ARHGEF18 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HS14171

YKO-HS14171(HeLa)
ARHGEF18 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HS14170

YKO-HS14170(HCT 116)

Related Popular Products

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

ARHGEF18 Knockout cell line (Hep G2)

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HT3981

inquiry More details
ARHGEF18 Knockout cell line (HeLa)

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HS14171

inquiry More details
ARHGEF18 Knockout cell line (HCT 116)

Organism: Human

Gene Name: ARHGEF18

Gene ID: 23370

Size: 1*10^6

Catalog#: YKO-HS14170

inquiry More details

ARHGEF18 Knockout cell line (IPSC)

Subscribe Us